MOLECULAR-MASS DISTRIBUTION OF CASEIN PHOSPHOPEPTIDES

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Review of casein phosphopeptides-amorphous calcium phosphate.

Casein phosphopeptides-amorphous calcium phosphate (CPP-ACP) is a bioactive agent with a base of milk products, which has been formulated from two parts: casein phosphopeptides (CPP) and amorphous calcium phosphate (ACP). CPP was produced from milk protein casein and has a remarkable ability to stabilize calcium phosphate in solution and to substantially increase the level of calcium phosphate ...

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Casein phosphopeptides from casein micelles by successive digestion with pepsin and trypsin.

When milk is ingested, casein micelles will be successively digested by pepsin in the stomach and trypsin in the intestine. Therefore, we digested casein micelles successively with pepsin at pH 4.0 and trypsin at pH 7.0, and recovered casein phosphopeptides (CPP) as CPP-calcium phosphate (CP) complexes. The CPP-CP complexes contained 248 mg of calcium/g peptides and 175 mg of inorganic phosphor...

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Intestinal absorption of calcium in rats given diets containing casein or amino acid mixture: the role of casein phosphopeptides.

1. In an attempt to investigate calcium absorption in the rat during the postprandial period, with the least alteration of the physical environment, the undisturbed small intestine was ligated in situ 2.5 or 3.0 h after ingestion of a diet containing 200 g casein/kg or an equivalent amino acid mixture, or 925 g casein/kg. Estimation of Ca absorption was made by comparing the amount of soluble 4...

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Characterisation of phosvitin phosphopeptides using MALDI-TOF mass spectrometry.

Putative phosphopeptides produced from enzyme hydrolysis of phosvitin were identified and characterised using MALDI-TOF/MS. Phosvitin was heat-pretreated and then hydrolysed using pepsin, thermolysin, and trypsin at their optimal pH and temperature conditions with or without partial dephosphorylation. Pepsin and thermolysin were not effective in producing phosphopeptides, but trypsin hydrolysis...

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Indirect effect of casein phosphopeptides on calcium absorption in rat ileum in vitro.

Casein phosphopeptides (CPP) are the phosphorylated fragments of bovine milk casein. They are believed to enhance intestinal absorption of calcium by their ability to form soluble complexes with calcium thereby inhibiting the precipitation of phosphate-calcium salts. In order to evaluate whether they also act in an additional direct manner on the intestinal mucosa, these peptides were added in ...

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ژورنال

عنوان ژورنال: Scientific Works of National University of Food Technologies

سال: 2018

ISSN: 2225-2924,2617-1945

DOI: 10.24263/2225-2924-2018-24-5-3